About ClearLine Diagnostic

Professional drug testing & diagnostic solutions in Illinois — DOT certified, CLIA-waived, and committed to accuracy in every collection.

CLIA
Waived Certified Facility
"Accurate, court-ready testing —
built on professionalism and trust."
DOT Certified
5-Star Service

Built on Professionalism and Compliance

At ClearLine Diagnostic, we are committed to delivering accurate, reliable, and confidential testing services that empower individuals, families, and organizations to make informed decisions with confidence. Specializing in drug testing and DNA testing, we combine advanced technology with industry best practices to provide fast, precise, and legally compliant results.

Our mission is simple: to bring clarity where it matters most. Whether you are an employer ensuring a safe and compliant workplace, a healthcare provider seeking dependable lab support, or an individual in need of personal testing services, ClearLine Diagnostic stands as a trusted partner every step of the way.

We offer a comprehensive range of services, including:

  • DOT and Non-DOT Drug Testing
  • Pre-employment, random, and post-accident testing
  • DNA testing — paternity, relationship, and legal
  • Mobile and on-site testing for businesses
  • Fast turnaround with secure, confidential reporting

At the core of our operations is a commitment to accuracy, integrity, and professionalism. Our trained and certified staff follow strict chain-of-custody procedures and adhere to all federal and industry regulations to ensure every test result is dependable and defensible.

What sets us apart is our focus on client experience. We understand that testing services often come at critical and sensitive moments, which is why we prioritize clear communication, respect, and discretion in every interaction.

As we continue to grow, ClearLine Diagnostic remains dedicated to innovation, expanding our services, and building long-term relationships with our clients and partners. We are proud to serve our community with solutions that promote safety, trust, and peace of mind.

DOT & Non-DOT Certified
CLIA-Waived Facility
9+ Service Types
Mobile Testing Available

Our Core Values

These principles guide every decision we make — from laboratory protocols to patient interactions.

Compliance First

Every collection follows DOT, FMCSA, and chain-of-custody standards. We ensure your results are defensible, court-ready, and legally compliant.

Certified Professionals

Our trained collectors maintain the highest standards for specimen handling, documentation, and confidentiality in every interaction.

Trusted & Transparent

Clear pricing, honest communication, and professional service — always. We treat every client with discretion, dignity, and respect.

100+
Clients Served
9+
Service Types
3
Counties Served
100%
Chain-of-Custody

Our Accreditations & Certifications

Independent validations of our quality, accuracy, and commitment to international diagnostic standards.

CLIA-Waived Certified
Clinical Laboratory Improvement Amendments waived facility for rapid medical testing
DOT Certified
Department of Transportation compliant drug & alcohol testing per 49 CFR Part 40
FMCSA Compliant
Federal Motor Carrier Safety Administration drug and alcohol regulations compliance
State Licensed (IL)
Properly licensed and operating in compliance with Illinois state regulations

Testing You Can Count On

Join hundreds of clients across South Illinois who trust ClearLine for accurate, court-ready drug testing and diagnostic services.

Book a Test Our Services